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This paper appraises an HPLC method for assaying phospholipase A2 (PLA2).The procedure is based on heptane-isopropanol-H2SO4 extraction of fatty acids released by the enzyme in the presence of margaric acid as an internal standard, and precolumn derivatization with 9-anthryldiazomethane.The derivatives of naturally occurring long-chain fatty acids
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